K5 Heparan Lyase

This stable enzyme cleaves heparan sulfate in the non-sulfated regions of the polymer chain. This action may release activities that lie cryptic in the intact heparan sulphate chain.

An excellent substrate for this enzyme is K5 polysaccharide (K5001) which can be used to monitor the progress of the enzyme action by measuring absorbance at 232nm.

K5 Heparan Lyase can be used to remove heparan sulfate from proteoglycans and it is the only enzymatic method available for excising the entire sulfated regions from heparan sulfate chains. It differs from heparinase 111 (heparitinase) in that it will not degrade the transition zones that have an intermediate level of sulfation. (Murphy et al., J. Biol. Chem. 279, 27239-27245 2004).

For information on regarding the action, substrate specificity and potential applications of K5 Heparan Lyase please download our Technical Bulletin – K5 Heparan Lyase.